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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been reported but detailed nucleotide sequence information and facts just isn't yet readily available.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guidebook outlines further possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guide outlines even further possible optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope around the target protein. By evaluating the staining from the blocked antibody versus the antibody by itself, one can see which staining is particular.
Target Information Cyclin E belongs to your hugely conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which add towards the temporal coordination of every mitotic party. Cyclin E types a complex with and functions for a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is particularly degraded as cells progress by way of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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